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TB-500 is a synthetic fraction of the protein thymosin beta-4, which is present in virtually all of human and animal cells. The primary purpose of this peptide is to promote healing. It also promotes creation of new muscle and blood cells. The healing effects of TB-500 have been observed in ligaments, tendons, muscle, skin, heart, and eyes. Thymosin beta-4 is naturally produced in higher concentration where the tissue has been damaged. This peptide is also a very potent anti-inflammatory agent.

TB-500 is different from other repair factors (like growth hormone) because it promotes keratinocyte  and endothelial migration. It also does not bind to the extracellular matrix and has an extremely low molecular weight.  Due to this, it can travel long distances through the tissues in the human body.

One of TB-500 key mechanisms of action is its ability to regulate the cell-building protein called ACTIN.  Of the thousands of proteins present within human cells, ACTIN represents roughly ten percent of the total. Therefore it is a vital component of cell structure and movement.